Description
This ELISA is designed for the quantitative determination of thymosin ß4 (Tß4) in serum and thymus preparations. The test principle is based on a competition between antigen in the sample or standards and the antigen coated on the wells of microplate. A peroxidase-conjugated antibody is used for detection and quantification, and tetramethylbenzidine (TMB) as a peroxidase substrate. The enzymatic reaction is terminated by acidic stop solution. The intensity of the colour is inversely proportional to the concentration of Tß4. A dose response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from standard. Tß4, present in the samples, is determined directly from this curve